What do biologists want? It's probably best to ask them.

Just a thought: more than likely, if a biologist wants to learn something, they'll need a tool that is specialized for measuring exactly the quantity that they're interested in. A general measurement tool will almost always be less-than-ideal for measuring some specific quantity. Therefore, we optical scientists should spend less effort in optimizing a mature imaging technology and instead work routinely with biologists to help solve their problems.